T-2 toxin, and its metabolite HT-2 toxin, may be found in cereal grains, particularly in oats. These mycotoxins are produced by Fusarium, which grows optimally in moist, cool conditions. Research is ongoing to evaluate the health effects of these toxins on human and animal health; however, researchers have shown that T-2 and HT-2 toxins are a concern for swine and poultry.

The ROSA T2/HT2 test enables grain and feed producers to quantitatively detect T-2 and HT-2 toxins. The Rapid One Step Assay is a lateral flow test that is read in the Charm EZ-M system or the ROSA-M Reader. Samples are extracted with 70% methanol.

The ROSA T2/HT2 test has been validated by Charm for detecting T-2 and HT-2 toxins in the following commodities: